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Distribution host: Escherichia coli DH5alpha

Size (kb): 4.898

DESCRIPTION OF VECTOR:
Intact vector size: 4.898
Type of vector: phagemid
Cloning sites: KpnI ApaI XhoI SalI ClaI EcoRI PstI SmaI BamHI SpeI EagI
NotI SacII SacI NaeI
Polylinker sites: BssHII KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI
SmaI BamHI SpeI XbaI EagI NotI BstXI SacII SacI BssHII
Construction: pBluescriptII KS+
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->, 417-1219
replicon: f1, <-, 1351-1807
insert detection: lacZ', <-, 1800-2137
promoter for in vitro transcription: T7, ->, 1977-1996
MCS: KpnI...SacI, ->, 2004-2111
promoter for in vitro transcription: T3, <-, 2123-2142
promoter: lac, <-, 2212-2240
replicon: pMB1, 2566-2566
marker(s): ampR, <-, 3324-4184
centromere: CEN6, 4326-4442
replicon: ARSH4, 4443-4806

Vector: pRS416 (phagemid)

Promoters: Promoter for in vitro transcription T7

Construction: pBluescriptII KS+

Marker(s):URA3,ampR

Construct size (kb): 4.898

Features: insert detection: lacZ'
marker(s): URA3
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARSH4
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
centromere: CEN6

YC-type (centromeric) shuttle vector

Restriction digests of the clone give the following sizes (kb): PvuII--4.45, 0.45; BglI--3.2, 1.7; PstI--3.25, 1.65.

A YC-type shuttle vector with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites.

Temperature: 37.0°C

component of:ATCC 87534