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pUR18
pUR18
規(guī)格:
貨期:
編號(hào):B244381
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pUR18
商品貨號(hào) B244381
Designations pUR18
Depositors NC Barbet, AM Carr
Biosafety Level 1
Vector Information
Size (kb): 5.5000000000000000
Vector: pUR18 (plasmid)
Promoters: Promoter lac
Construction: pUC18, pON163
Marker(s):ampR,ura4+
Construct size (kb): 5.5
Features: insert detection: lacZ'
marker(s): ampR
marker(s): ura4+
promoter: lac
replicon: ars1
replicon: pMB1
Applications
expression vector
shuttle vector
vector permitting construction of fusion proteins
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.5; BamHI--5.5; PstI--5.5; HindIII--5.5.
Shuttle vector permitting visual detection of recombinants by beta-galactosidase alpha complementation.
Constructed by inserting a 1.7 kb ClaI fragment containing ura4 and a 1.2 kb ClaI fragment containing ars1 into the NdeI site modified with a ClaI linker.
The order of the major features in this plasmid is: ars1 - ura4 - HindIII/MCS/EcoRI/lacZ' - pMB1 ori - ampR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Barbet N, et al. Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe. Gene 114: 59-66, 1992. PubMed: 1587485

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